Application
Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of VPS53 in 293 cells with VPS53 antibody at 2.5 μg/mL.Application
Immunocytochemistry of VPS53 in 293 cells with VPS53 antibody at 5 μg/mL.| Product Name | VPS53 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VPS53 Antibody: HCCS1, hVps53L, pp13624, Vacuolar protein sorting-associated protein 53 homolog |
| Product description | VPS53 Antibody: The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.1) Pfeffer SR. Targeting of proteins to the lysosome. Curr. Top. Microbiol. Immunol.1991; 170:43-65. |
| Immunogen | VPS53 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human VPS53. The immunogen is located within the last 50 amino acids of VPS53. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VPS53 |
|---|---|
| Alternative Names | VPS53 Antibody: HCCS1 hVps53L pp13624 Vacuolar protein sorting-associated protein 53 homolog |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
Application
Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of VPS53 in 293 cells with VPS53 antibody at 2.5 μg/mL.
Application
Immunocytochemistry of VPS53 in 293 cells with VPS53 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VPS53 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | VPS53 Antibody is supplied in PBS containing 0.02% sodium azide. |
