Application
Western blot analysis of VTI1a in human brain tissue lysate with VTI1a antibody at 1 μg/ml.Application
Immunohistochemistry of VTI1a in human brain tissue with VTI1a antibody at 5 μg/mL.| Product Name | VTI1a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). VTI1a mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane and promotes fusion of the lipid bilayers (4). VTI1a may be concerned with increased secretion of cytokines associated with cellular senescence (5).1) Fischer VM and Stevens TH. A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways. J. Biol. Chem. 1998; 273:2624-30. |
| Immunogen | VTI1a antibody was raised against a 19 amino acid peptide near the center of human VTI1a. The immunogen is located within amino acids 50 - 100 of VTI1a. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| VTI1a antibody can be used for detection of VTI1a by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VTI1a |
|---|---|
| Alternative Names | Vesicle transport through interaction with t-SNAREs 1A MMDS3 MVti1 VTI1RP2 Vti1-rp2 |
| Molecular Weight(MW) | Predicted: 24, 27 kDa Observed: 26 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | VTI1a antibody is human, mouse and rat reactive. At least two isoforms of VTI1a are known to exist; this antibody will detect both isoforms. VTI1a antibody is predicted to not cross-react with VTI1b. |
Application
Western blot analysis of VTI1a in human brain tissue lysate with VTI1a antibody at 1 μg/ml.
Application
Immunohistochemistry of VTI1a in human brain tissue with VTI1a antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | VTI1a antibody can be used for detection of VTI1a by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | VTI1a antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | VTI1a antibody is supplied in PBS containing 0.02% sodium azide. |
