Application
Western blot analysis of VTI1b in HeLa cell lysate with VTI1b antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of VTI1b in human brain tissue with VTI1b antibody at 5 μg/mL.| Product Name | VTI1b Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). It is thought that VTI1b along with the SNARE protein VAMP8 mediates the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential process for autophagy (4).1) Fischer VM and Stevens TH. A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways. J. Biol. Chem. 1998; 273:2624-30. |
| Immunogen | VTI1b antibody was raised against a 17 amino acid peptide near the center of human VTI1b. The immunogen is located within amino acids 150 - 200 of VTI1b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| VTI1b antibody can be used for detection of VTI1b by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VTI1b |
|---|---|
| Alternative Names | Vesicle transport through interaction with t-SNAREs 1B VTI1 VTI1-LIKE VTI1L VTI2 v-SNARE vti1-rp1 |
| Molecular Weight(MW) | Predicted: 14, 19, 26 kDa Observed: 27 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | VTI1b antibody is human, mouse and rat reactive. At least three isoforms of VTI1a are known to exist; this antibody will detect all three isoforms. VTI1b antibody is predicted to not cross-react with VTI1a. |
Application
Western blot analysis of VTI1b in HeLa cell lysate with VTI1b antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of VTI1b in human brain tissue with VTI1b antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | VTI1b antibody can be used for detection of VTI1b by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | VTI1b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | VTI1b antibody is supplied in PBS containing 0.02% sodium azide. |
