Application
Overlay histogram showing MCF-7 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IApplication
Western Blot at 1:32000 dilution + human plasma lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:8000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with VTN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | VTN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.1) Jenne D.E., Stanley K.K.EMBO J. 4:3153-3157(1985) Sigurdardottir O., Wiman B.Biochim. Acta 1208:104-110(1994) Seiffert D., Loskutoff D.J.J. Biol. Chem. 266:2824-2830(1991) |
| Immunogen | This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the N-terminal region of human VTN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACS,IHC-P,WB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:8000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VTN |
|---|---|
| Alternative Names | Vitronectin VN S-protein Serum-spreading factor V75 Vitronectin V65 subunit Vitronectin V10 subunit Somatomedin-B VTN |
| Molecular Weight(MW) | 54 kDa |
Application
Overlay histogram showing MCF-7 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I
Application
Western Blot at 1:32000 dilution + human plasma lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:8000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with VTN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:8000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
