Application
Western blot analysis of WAC in human testis tissue lysate with WAC antibody at 1 μg/mL.Application
Immunofluorescence of WAC in mouse testis tissue with WAC antibody at 5 μg/ml.Application
Immunohistochemistry of WAC in mouse testis tissue with WAC antibody at 2.5 μg/ml.| Product Name | WAC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WAC Antibody: Wwp4, BM-016, PRO1741, KIAA1844, WW domain-containing adapter protein with coiled-coil |
| Product description | WAC Antibody: The WW domain containing adaptor with coiled-coil protein (WAC) contains a WW domain that mediates protein-protein interactions and colocalizes with RNA splicing factor SC35. Further studies have indicated that WAC is a functional partner of the RNF20/40 complex that ubiquitinates Histone H2B, and that WAC regulates H2B ubiquitination. WAC targets RNF20/40 to associate with RNA polymerase II complex for H2B ubiquitination at active transcription sites. WAC-dependent transcription is also important for cell-cycle checkpoint activation in response to genotoxic stress.1) Xu GM and Arnaout MA. WAC, a novel WW domain-containing adapter with a coiled-coil region, is colocalized with splicing factor SC35. Genomics 2002; 79:87-94. |
| Immunogen | WAC antibody was raised against a 19 amino acid peptide near the center of human WAC . The immunogen is located within amino acids 210 - 260 of WAC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WAC Antibody can be used for detection of WAC by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WAC |
|---|---|
| Alternative Names | WAC Antibody: Wwp4 BM-016 PRO1741 KIAA1844 WW domain-containing adapter protein with coiled-coil |
| Molecular Weight(MW) | Predicted: 71 kDa Observed: 75 kDa |
| Tissue Specificity | At least two isoforms of WAC are known to exist; this antibody will detect the larger isoform. |
Application
Western blot analysis of WAC in human testis tissue lysate with WAC antibody at 1 μg/mL.
Application
Immunofluorescence of WAC in mouse testis tissue with WAC antibody at 5 μg/ml.
Application
Immunohistochemistry of WAC in mouse testis tissue with WAC antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | WAC Antibody can be used for detection of WAC by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WAC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | WAC Antibody is supplied in PBS containing 0.02% sodium azide. |
