Application
Western blot analysis of WAPL in A20 cell lysate with WAPL antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of WAPL in rat heart tissue with WAPL antibody at 20 μg/mL.Application
Immunohistochemistry of WAPL in rat heart tissue with WAPL antibody at 5 μg/mL.| Product Name | WAPL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WAPL Antibody: FOE, WAPL, KIAA0261, FOE, Wings apart-like protein homolog, Friend of EBNA2 protein |
| Product description | WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1). It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1,2,4). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3,4). WAPL is a new regulator of the development and metastasis of cancerous tissue (4,5).1) Kwiatkowski BA, Ragoczy T, Ehly J, et al. Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Exp. Cell Res. 2004; 300:223-33. |
| Immunogen | WAPL antibody was raised against an 18 amino acid peptide near the amino terminus of human WAPL. The immunogen is located within amino acids 30 - 80 of WAPL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WAPAL |
|---|---|
| Alternative Names | WAPL Antibody: FOE WAPL KIAA0261 FOE Wings apart-like protein homolog Friend of EBNA2 protein |
| Molecular Weight(MW) | Predicted: 131 kDa Observed: 140 kDa |
| Tissue Specificity | WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist. |
Application
Western blot analysis of WAPL in A20 cell lysate with WAPL antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of WAPL in rat heart tissue with WAPL antibody at 20 μg/mL.
Application
Immunohistochemistry of WAPL in rat heart tissue with WAPL antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WAPL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | WAPL antibody is supplied in PBS containing 0.02% sodium azide. |
