Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
WDR12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | WDR12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ribosome biogenesis protein WDR12 {ECO:0000255|HAMAP-Rule:MF_03029}, WD repeat-containing protein 12 {ECO:0000255|HAMAP-Rule:MF_03029}, WDR12 {ECO:0000255|HAMAP-Rule:MF_03029} |
| Product description | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein is highly similar to the mouse WD repeat domain 12 protein at the amino acid level. The protein encoded by this gene is a component of a nucleolar protein complex that affects maturation of the large ribosomal subunit.1) Kathiresan, S., et al. Nat. Genet. 41(3):334-341(2009) |
| Immunogen | This WDR12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from the Central region of human WDR12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WDR12 |
|---|---|
| Alternative Names | Ribosome biogenesis protein WDR12 {ECO:0000255|HAMAP-Rule:MF_03029} WD repeat-containing protein 12 {ECO:0000255|HAMAP-Rule:MF_03029} WDR12 {ECO:0000255|HAMAP-Rule:MF_03029} |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
WDR12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
