Application
Western blot analysis of WDR18 in rat lung tissue lysate with WDR18 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of WDR18 in human lung tissue with WDR18 antibody at 2.5 μg/mL.| Product Name | WDR18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WDR18 Antibody: Ipi3, R32184_1, WD repeat-containing protein 18 |
| Product description | WDR18 Antibody: WD repeat domain 18 (WDR18) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeats are minimally conserved regions of approximately 40 amino acids that commonly form a tertiary propeller structure. WDR18 is a 432 amino acid protein that contains six WD repeats and is encoded by a gene located on human chromosome 19. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit.1) Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5. |
| Immunogen | WDR18 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WDR18. The immunogen is located within the last 50 amino acids of WDR18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| WDR18 antibody can be used for detection of WDR18 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WDR18 |
|---|---|
| Alternative Names | WDR18 Antibody: Ipi3 R32184_1 WD repeat-containing protein 18 |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | WDR18 antibody is predicted to not cross-react with other WDR family members. |
Application
Western blot analysis of WDR18 in rat lung tissue lysate with WDR18 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of WDR18 in human lung tissue with WDR18 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
|---|---|
| Application Notes | WDR18 antibody can be used for detection of WDR18 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WDR18 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | WDR18 Antibody is supplied in PBS containing 0.02% sodium azide. |
