Application
Western blot analysis in A549 cell line lysates (35ug/lane).Application
WDR32 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WDR32 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDB1- and CUL4-associated factor 10, WD repeat-containing protein 32, DCAF10, WDR32 |
| Product description | DCAF10 may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.1) Jin, J., et al. Mol. Cell 23(5):709-721(2006) |
| Immunogen | This WDR32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-142 amino acids from the N-terminal region of human WDR32. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCAF10 |
|---|---|
| Alternative Names | DDB1- and CUL4-associated factor 10 WD repeat-containing protein 32 DCAF10 WDR32 |
| Molecular Weight(MW) | 61 kDa |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).
Application
WDR32 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
