Application
Western blot analysis of WDR61 in SK-N-SH cell lysate with WDR61 antibody at 1 μg/ml.Application
Immunofluorescence of WDR61 in mouse brain tissue with WDR61 antibody at 20 μg/ml.Application
Immunohistochemistry of WDR61 in mouse brain tissue with WDR61 antibody at 5 μg/ml.| Product Name | WDR61 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WD repeat-containing protein 61, Meiotic recombination REC14 protein homolog, REC14, SKI8 |
| Product description | WDR61 (WD-repeat-containing protein 61), also known as SKI8 or REC14, is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (1,2). PAF1C is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C (3,4).1) Zhu B, Mandal SS, Pham AD, et al. The human PAF complex coordinates transcription with events downstream of RNA synthesis. Genes Dev. 2005; 19:1668-73. |
| Immunogen | WDR61 antibody was raised against a 14 amino acid peptide near the amino terminus of human WDR61. The immunogen is located within the first 50 amino acids of WDR61. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WDR61 antibody can be used for detection of WDR61 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WDR61 |
|---|---|
| Alternative Names | WD repeat-containing protein 61 Meiotic recombination REC14 protein homolog REC14 SKI8 |
| Molecular Weight(MW) | Predicted: 34 kDa Observed: 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (93%), Chicken: (79%) |
| Tissue Specificity | WDR61 antibody is human, mouse and rat reactive. |
Application
Western blot analysis of WDR61 in SK-N-SH cell lysate with WDR61 antibody at 1 μg/ml.
Application
Immunofluorescence of WDR61 in mouse brain tissue with WDR61 antibody at 20 μg/ml.
Application
Immunohistochemistry of WDR61 in mouse brain tissue with WDR61 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | WDR61 antibody can be used for detection of WDR61 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WDR61 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | WDR61 antibody is supplied in PBS containing 0.02% sodium azide. |
