Application
Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WFDC1 antibody detected the WFDC1 protein (arrow).Application
immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WFDC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WAP four-disulfide core domain protein 1, Prostate stromal protein ps20, ps20 growth inhibitor, WFDC1, PS20 |
| Product description | This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. The encoded protein shares 81% amino acid identity with the rat ps20 protein, which was originally identified as a secreted growth inhibitor. This gene is mapped to chromosome 16q24, an area of frequent loss of heterozygosity in cancers, including prostate, breast and hepatocellular cancers and Wilms' tumor. Owing to its location and a possible growth inhibitory property of its gene product, this gene is suggested to be a tumor suppressor gene.1) Briggs, F.B., et al. Genes Immun. 11(3):199-208(2010) |
| Immunogen | This WFDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-177 amino acids from the C-terminal region of human WFDC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WFDC1 |
|---|---|
| Alternative Names | WAP four-disulfide core domain protein 1 Prostate stromal protein ps20 ps20 growth inhibitor WFDC1 PS20 |
| Molecular Weight(MW) | 24 kDa |
Application
Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the WFDC1 antibody detected the WFDC1 protein (arrow).
Application
immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
