Application
Western blot analysis of WIPI1 in rat colon tissue lysate with WIPI1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of WIPI1 in human colon tissue with WIPI1 antibody at 20 μg/mL.| Product Name | WIPI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WIPI1 Antibody: ATG18, ATG18A, WIPI49, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog, WIPI-1 |
| Product description | WIPI1 Antibody: WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as WIPI1, ATG18 or WIPI49, is thought to play a role in autophagy and may regulate protein trafficking in certain recycling pathways. It contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles and the trans-Golgi network. It is ubiquitously expressed with highest expression in heart, testis, placenta, pancreas and skeletal muscle. WIPI1 is upregulated in a variety of tumors, suggesting a role in carcinogenesis.1) Proikas-Cezanne T and Robenek H. Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J. Cell. Mol. Med. 2011; 15:2007-10. |
| Immunogen | WIPI1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WIPI1. The immunogen is located within amino acids 350 - 400 of WIPI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| WIPI1 antibody can be used for detection of Wipi1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WIPI1 |
|---|---|
| Alternative Names | WIPI1 Antibody: ATG18 ATG18A WIPI49 WD repeat domain phosphoinositide-interacting protein 1 Atg18 protein homolog WIPI-1 |
| Molecular Weight(MW) | 49 kDa |
| Tissue Specificity | At least two isoforms of WIPI1 are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of WIPI1 in rat colon tissue lysate with WIPI1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of WIPI1 in human colon tissue with WIPI1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1472 - Rat Colon Tissue Lysate |
|---|---|
| Application Notes | WIPI1 antibody can be used for detection of Wipi1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WIPI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | WIPI1 Antibody is supplied in PBS containing 0.02% sodium azide. |
