WB
Western blot analysis of WIPI1 on different lysates with Rabbit anti-WIPI1 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-WIPI1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | WIPI1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human WIPI1 aa 20-120. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | WIPI1 |
|---|---|
| Gene Synonyms | ATG18 ATG18A WIPI49 |
| Gene Full Name | WD repeat domain, phosphoinositide interacting 1 |
| Gene Summary | This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| Alternative Names | ATG 18 antibody ATG18 antibody Atg18 protein homolog antibody ATG18A antibody FLJ10055 antibody WD repeat domain phosphoinositide interacting 1 antibody WD repeat domain phosphoinositide interacting protein 1 antibody WD repeat domain phosphoinositide-interacting protein 1 antibody WD40 repeat protein interacting with phosphoinositides of 49 kDa antibody WD40 repeat protein interacting with phosphoInositides of 49kDa antibody WIPI 1 alpha antibody WIPI 1 antibody WIPI 49 antibody WIPI 49 kDa antibody WIPI-1 antibody wipi1 antibody WIPI1_HUMAN antibody WIPI49 antibody |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Endosome, trans-Golgi network, cytoskeleton, clathrin-coated vesicle, preautophagosomal structure membrane. |
WB
Western blot analysis of WIPI1 on different lysates with Rabbit anti-WIPI1 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-WIPI1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
