Application
Western blot analysis of WIPI2 in human testis tissue lysate with WIPI2 antibody at 1 μg/mL| Product Name | WIPI2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WIPI2 Antibody: Atg21, ATG18B, CGI-50, WIPI-2, WD repeat domain phosphoinositide-interacting protein 2, WIPI49-like protein 2 |
| Product description | WIPI2 Antibody: WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.1) Smith TF. Diversity of WD-repeat proteins. Subcell. Biochem. 2008; 48:20-30. |
| Immunogen | Rabbit polyclonal WIPI2 antibody was raised against a 17 amino acid peptide near the amino terminus of human WIPI2. The immunogen is located within the first 50 amino acids of WIPI2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WIPI2 |
|---|---|
| Alternative Names | WIPI2 Antibody: Atg21 ATG18B CGI-50 WIPI-2 WD repeat domain phosphoinositide-interacting protein 2 WIPI49-like protein 2 |
| Molecular Weight(MW) | 50 kDa |
| Tissue Specificity | WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1. |
Application
Western blot analysis of WIPI2 in human testis tissue lysate with WIPI2 antibody at 1 μg/mL| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WIPI2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | WIPI2 Antibody is supplied in PBS containing 0.02% sodium azide. |
