Application
Western blot analysis of anti-WISP2 Antibody in A549 cell lysateApplication
WISP2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | WISP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Wisp2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. Wisp2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.1) Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Banerjee, S., et al., Neoplasia 5(1):63-73 (2003). Kumar, S., et al., J. Biol. Chem. 274(24):17123-17131 (1999). Pennica, D., et al., Proc. Natl. Acad. Sci. U.S.A. 95(25):14717-14722 (1998). Saxena, N., et al., Mol. Cell. Biochem. 228 (1-2), 99-104 (2001). |
| Immunogen | This WISP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-116 amino acids from the Central region of human WISP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | WISP2 |
|---|---|
| Alternative Names | WNT1-inducible-signaling pathway protein 2 WISP-2 CCN family member 5 Connective tissue growth factor-like protein CTGF-L Connective tissue growth factor-related protein 58 WISP2 CCN5 CT58 CTGFL |
| Molecular Weight(MW) | 27 kDa |
Application
Western blot analysis of anti-WISP2 Antibody in A549 cell lysate
Application
WISP2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
