Application
Western blot analysis of WIZ in rat lung tissue lysate with WIZ antibody at 1 μg/mL.Application
Immunofluorescence of WIZ in human brain tissue with WIZ antibody at 20 μg/mL.Application
Immunohistochemistry of WIZ in human brain tissue with WIZ antibody at 2.5 μg/mL.| Product Name | WIZ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WIZ Antibody: ZNF803, ZNF803, Protein Wiz, Widely-interspaced zinc finger-containing protein |
| Product description | WIZ Antibody: WIZ, also known as ZNF803, was initially identified in a mouse cerebellum cDNA library screen and its message was found to be expressed in the granule cell layers of the cerebellum as well as in the dentate gyrus and olfactory bulb. Later analysis indicates however that WIZ is ubiquitously expressed. WIZ is a nuclear protein that co-localizes with G9a, a histone methyltransferase responsible for mono- and dimethylation of H3K9 at euchromatic regions. WIZ can also associate with CtBP family proteins, known to be transcriptional co-repressors, and has been suggested to link G9a/GLP complexes to the CtBP co-repressor machinery, possibly regulating complex stability and gene silencing.1) Matsumoto K, Ishii N, Yoshida S, et al. Molecular cloning and distinct developmental expression pattern of spliced forms of a novel zinc finger gene wiz in the mouse cerebellum. Brain Res. Mol. Brain Res.1998; 61:179-89. |
| Immunogen | WIZ antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TFF3. The immunogen is located within the last 50 amino acids of WIZ. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WIZ antibody can be used for detection of WIZ by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WIZ |
|---|---|
| Alternative Names | WIZ Antibody: ZNF803 ZNF803 Protein Wiz Widely-interspaced zinc finger-containing protein |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least four isoforms of WIZ are known to exist; this antibody will detect all four. |
Application
Western blot analysis of WIZ in rat lung tissue lysate with WIZ antibody at 1 μg/mL.
Application
Immunofluorescence of WIZ in human brain tissue with WIZ antibody at 20 μg/mL.
Application
Immunohistochemistry of WIZ in human brain tissue with WIZ antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
|---|---|
| Application Notes | WIZ antibody can be used for detection of WIZ by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WIZ antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | WIZ Antibody is supplied in PBS containing 0.02% sodium azide. |
