Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | WNK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase WNK1, 2.7.11.1, Erythrocyte 65 kDa protein, p65, Kinase deficient protein, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, hWNK1, WNK1, HSN2, KDP, KIAA0344, PRKWNK1 |
| Product description | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide- sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L.1) Verissimo F.,et al.Oncogene 20:5562-5569(2001). |
| Immunogen | This WNK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1848-1882 amino acids from human WNK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WNK1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase WNK1 2.7.11.1 Erythrocyte 65 kDa protein p65 Kinase deficient protein Protein kinase lysine-deficient 1 Protein kinase with no lysine 1 hWNK1 WNK1 HSN2 KDP KIAA0344 PRKWNK1 |
| Molecular Weight(MW) | 251 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
