Application
Western blot analysis of WNT5 in Jurkat cell lysate with WNT5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of WNT5 in rat colon tissue with WNT5 antibody at 5 μg/mL.| Product Name | WNT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Wingless-type MMTV integration site family member 5 |
| Product description | WNT5A and WNT5B are near-identical members of the WNT gene family, which consists of structurally related genes which encode secreted signaling proteins that have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis (1,2). WNT5A and WNT5B coordinate chondrocyte proliferation and differentiation by differentially regulating cyclin D1 and p130 expression, as well as chondrocyte-specific Col2a1 expression (3). Up-regulation of WNT5A and WNT5B has been observed in several types of human cancers, suggesting that they may be useful as diagnostic markers or potent therapeutic targets (4).1) Saitoh T and Katoh M. Molecular cloning and characterization of human WNT5B on chromosome 12p13.3 region. Int. J. Oncol. 2001; 19:347-51. |
| Immunogen | WNT5 antibody was raised against a 15 amino acid peptide near the center of human WNT5B. The immunogen is located within amino acids 130 - 180 of WNT5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WNT5 |
|---|---|
| Alternative Names | Wingless-type MMTV integration site family member 5 |
| Molecular Weight(MW) | Predicted: 39 kDa Observed: 465 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit: (93%) |
| Tissue Specificity | WNT5 antibody is human, mouse and rat reactive. WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of WNT5 in Jurkat cell lysate with WNT5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of WNT5 in rat colon tissue with WNT5 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WNT5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | WNT5 antibody is supplied in PBS containing 0.02% sodium azide. |
