Application
Cat. No. 1201 – HeLa Cell LysateApplication
Immunofluorescence of WSB1 in human colon tissue with WSB1 antibody at 20 μg/mL.Application
Immunohistochemistry of WSB1 in human colon tissue with WSB1 antibody at 5 μg/mL.| Product Name | WSB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1), is a member of the WD-protein subfamily (1). It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1,2). WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3). Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4,5).1) Vasiliauskas D, Hancock S, Stern CD. SWiP-1: novel SOCS box containing WD-protein regulated by signalling centres and by Shh during development. Mech. Dev. 1999; 82:79-94. |
| Immunogen | WSB1 antibody was raised against a 16 amino acid peptide near the amino terminus of human WSB1. The immunogen is located within amino acids 70 - 120 of WSB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WSB1 |
|---|---|
| Alternative Names | WD repeat and SOCS box containing 1 SOCS box-containing WD protein SWiP-1 SWIP1 WD repeat and SOCS box-containing protein 1 WSB-1 |
| Molecular Weight(MW) | Predicted: 46 kDa Observed: 46 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (82%) |
| Tissue Specificity | WSB1 antibody is human specific. |
Application
Cat. No. 1201 – HeLa Cell Lysate
Application
Immunofluorescence of WSB1 in human colon tissue with WSB1 antibody at 20 μg/mL.
Application
Immunohistochemistry of WSB1 in human colon tissue with WSB1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | WSB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | WSB1 antibody is supplied in PBS containing 0.02% sodium azide. |
