Application
Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody. The lane on the right is treated with the antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | WWOX (phospho Tyr33) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FOR, WOX1, Aberrant WW domain-containing oxidoreductase |
| Product description | WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism.1) Bednarek A.K., Cancer Res. 60:2140-2145(2000). |
| Immunogen | WWOX (phospho Tyr33) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y (p)-Y-A) derived from Human WWOX. |
| Modification | p-Tyr33 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WWOX |
|---|---|
| Alternative Names | FOR WOX1 Aberrant WW domain-containing oxidoreductase |
| Molecular Weight(MW) | 55 kDa |
| Tissue Specificity | The antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33. |
| Entrez Gene | 51741 |
|---|---|
| Protein Accession | NP_057457.1 |
Application
Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody. The lane on the right is treated with the antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
