Application
Western blot analysis of XAF-1 in human brain tissue lysate with XAF-1 antibody at (A) 0.5 and (B) 1 μg/mL..Application
Immunofluorescence of XAF-1 in Human Spleen cells with XAF-1 antibody at 20 μg/mL.| Product Name | XAF-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XAF-1 Antibody: BIRC4BP, XIAPAF1, HSXIAPAF1, BIRC4BP, XIAP-associated factor 1, BIRC4-binding protein |
| Product description | XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.1) Liston P, Fong W, Kelly NL, et al. Identification of XAF1 as an antagonist of XIAP anticaspase activity. Nature Cell Biol. 2001;3:128-33. |
| Immunogen | XAF-1 antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1. The immunogen is located within amino acids 230 - 280 of XAF-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 - 1 μg/mL. Multiple isoforms of XAF-1 exist and a doublet at ~30 kDa can be seen using anti-XAF-1 (IN). For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XAF1 |
|---|---|
| Alternative Names | XAF-1 Antibody: BIRC4BP XIAPAF1 HSXIAPAF1 BIRC4BP XIAP-associated factor 1 BIRC4-binding protein |
| Molecular Weight(MW) | Predicted: 31, 33 kDa Observed: 31 kDa |
Application
Western blot analysis of XAF-1 in human brain tissue lysate with XAF-1 antibody at (A) 0.5 and (B) 1 μg/mL..
Application
Immunofluorescence of XAF-1 in Human Spleen cells with XAF-1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 - 1 μg/mL. Multiple isoforms of XAF-1 exist and a doublet at ~30 kDa can be seen using anti-XAF-1 (IN). For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | XAF-1antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | XAF-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM295218