Application
Western blot analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 μg/mL.Application
Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 2 μg/mL.Application
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/mL.| Product Name | XBP-1 Antibody [9B7E5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | XBP-1 Monoclonal Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.1) Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91. |
| Immunogen | Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISA,ICC,IF,WB |
| XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XBP1 |
|---|---|
| Alternative Names | XBP-1 Antibody [9B7E5] : XBP2 TREB5 XBP-1 |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
Application
Western blot analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 μg/mL.
Application
Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 2 μg/mL.
Application
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 95-109 - XBP-1 Recombinant Protein |
|---|---|
| Application Notes | XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | XBP-1 monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
