Application
Antibody used in IHC on Human Pancreas at none (Lane 2: 15ug MCF7 lysate).Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1. 30 ug MiaPaca-2 cell lysate, Lane 2. 30 ug Panc-1 cell lysate).Application
Antibody used in IHC on Human A549 at 1:100.Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Product Name | XRCC5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XRCC5, KU80, KUB2, Ku86, NFIV, KARP1, KARP-1 |
| Product description | XRCC5 encodes the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V (D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V (D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.1) Sawchuk, D.J., et al., (2004) J. Biol. Chem. 279 (28), 29821-29831. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human XRCC5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| XRCC5 antibody can be used for detection of XRCC5 by ELISA at 1:12500. XRCC5 antibody can be used for detection of XRCC5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | XRCC5 |
|---|---|
| Alternative Names | XRCC5 KU80 KUB2 Ku86 NFIV KARP1 KARP-1 |
| Molecular Weight(MW) | 83 kDa |
Application
Antibody used in IHC on Human Pancreas at none (Lane 2: 15ug MCF7 lysate).
Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1. 30 ug MiaPaca-2 cell lysate, Lane 2. 30 ug Panc-1 cell lysate).
Application
Antibody used in IHC on Human A549 at 1:100.
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | XRCC5 antibody can be used for detection of XRCC5 by ELISA at 1:12500. XRCC5 antibody can be used for detection of XRCC5 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store XRCC5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
