Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.Application
Antibody used in IHC on Human Muscle.| Product Name | YAF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YAF2, MGC41856, |
| Product description | YAF2 interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. YAF2 contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1.The protein encoded by this gene interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two have been confirmed to date.1) Madge, B., (2003) Cancer Lett. 193 (2), 171-176. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YAF2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| YAF2 antibody can be used for detection of YAF2 by ELISA at 1:62500. YAF2 antibody can be used for detection of YAF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | YAF2 |
|---|---|
| Alternative Names | YAF2 MGC41856 |
| Molecular Weight(MW) | 20 kDa |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Application
Antibody used in IHC on Human Muscle.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | YAF2 antibody can be used for detection of YAF2 by ELISA at 1:62500. YAF2 antibody can be used for detection of YAF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store YAF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
