Application
Antibody used in WB on Mouse NIH-3T3 at 0.2-1 ug/ml.| Product Name | YAF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YAF2, 2810021M11Rik |
| Product description | Yaf2 binds to MYC and inhibits MYC-mediated transactivation. Yaf2 also binds to MYCN and enhances MYCN-dependent transcriptional activation. Yaf2 increases calpain 2-mediated proteolysis of YY1 in vitro. Yaf2 is a component of the E2F6.com-1 complex, a repressive complex that methylates Lys-9 of histone H3, suggesting that it is involved in chromatin-remodeling.1) Kaneko, T., Gene 315, 183-192 (2003). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse YAF2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| YAF2 antibody can be used for detection of YAF2 by ELISA at 1:312500. YAF2 antibody can be used for detection of YAF2 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YAF2 |
|---|---|
| Alternative Names | YAF2 2810021M11Rik |
| Molecular Weight(MW) | 20 kDa |
Application
Antibody used in WB on Mouse NIH-3T3 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | YAF2 antibody can be used for detection of YAF2 by ELISA at 1:312500. YAF2 antibody can be used for detection of YAF2 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store YAF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
