Application
Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | YB1 (phospho Ser102) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBF-A, NSEP1, EFI-A, MSY-1, YBX1 |
| Product description | YB-1 is a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP.1) Sakura H., Gene 73:499-507(1988). |
| Immunogen | YB1 (phospho Ser102) antibody was raised against a peptide sequence around phosphorylation site of Serine 102 (L-R-S (p)-V-G) derived from Human YB1. |
| Modification | p-Ser102 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YBX1 |
|---|---|
| Alternative Names | CBF-A NSEP1 EFI-A MSY-1 YBX1 |
| Molecular Weight(MW) | 36 kDa |
| Tissue Specificity | The antibody detects endogenous levels of YB1 only when phosphorylated at serine 102. |
| Entrez Gene | 4904 |
|---|---|
| Protein Accession | NP_004550.2 |
Application
Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
