Application
Western blot analysis in 293T cell line lysates (35ug/lane).Application
Fluorescent image of A549 cell stained with YBX1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/| Product Name | YBX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, DBPB, Enhancer factor I subunit A, EFI-A, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1, NSEP1, YB1 |
| Product description | Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. Regulates the transcription of numerous genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.1) Yu, Y.N., et al. Int. J. Oncol. 37(2):483-492(2010) |
| Immunogen | This YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-178 amino acids from the Central region of human YBX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YBX1 |
|---|---|
| Alternative Names | Nuclease-sensitive element-binding protein 1 CCAAT-binding transcription factor I subunit A CBF-A DNA-binding protein B DBPB Enhancer factor I subunit A EFI-A Y-box transcription factor Y-box-binding protein 1 YB-1 YBX1 NSEP1 YB1 |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in 293T cell line lysates (35ug/lane).
Application
Fluorescent image of A549 cell stained with YBX1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
