Application
Western blot analysis of lysate from U-251 MG cell line, using YBX1 Antibody at 1:1000.Application
Western blot analysis of YBX1 using rabbit polyclonal YBX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2).Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Fluorescent confocal image of U251 cell stained with YBX1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin| Product Name | YBX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, DBPB, Enhancer factor I subunit A, EFI-A, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1, NSEP1, YB1 |
| Product description | YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.1) Fujii,T., Cancer Res. 68 (5), 1504-1512 (2008) |
| Immunogen | This YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the C-terminal region of human YBX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | YBX1 |
|---|---|
| Alternative Names | Nuclease-sensitive element-binding protein 1 CCAAT-binding transcription factor I subunit A CBF-A DNA-binding protein B DBPB Enhancer factor I subunit A EFI-A Y-box transcription factor Y-box-binding protein 1 YB-1 YBX1 NSEP1 YB1 |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
Western blot analysis of lysate from U-251 MG cell line, using YBX1 Antibody at 1:1000.
Application
Western blot analysis of YBX1 using rabbit polyclonal YBX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2).
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Fluorescent confocal image of U251 cell stained with YBX1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
