Application
Western blot analysis of YPEL1 in Hela cell lysate with YPEL1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of YPEL1 in HeLa cells with YPEL1 antibody at 20 μg/mL.Application
Immunocytochemistry of YPEL1 in HeLa cells with YPEL1 antibody at 2.5 μg/mL.| Product Name | YPEL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | YPEL1 Antibody: YPEL1 (yippee-like 1) is thought to play a role in the epitheloid conversion of fibroblasts and the regulation of the cellular morphology. It is a 119 amino acid protein that localizes to the nucleus and belongs to a family of yippee-like proteins that include YPEL2 - 5, all of which localize to the centrosome and nucleus and are widely expressed in both adult and fetal tissue. The family of human YPEL proteins share a high degree of sequence homology with their rodent homologs, suggesting a conserved function between species. YPEL1 might be an important factor during the development and malignant transformation of tissues.1) Farlie P, Reid C, Wilcox S, et al. YPEL1: a novel nuclear protein that induces an epithelial-like morphology in fibroblasts. Genes Cells 2001; 6:619-29. |
| Immunogen | YPEL1 antibody was raised against a 11 amino acid synthetic peptide near the amino terminus of human YPEL1. The immunogen is located within the first 50 amino acids of YPEL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,ICC,IF,WB |
| YPEL1 antibody can be used for detection of YPEL1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YPEL1 |
|---|---|
| Alternative Names | YPEL1 Antibody: FKSG3 Protein yippee-like 1 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Western blot analysis of YPEL1 in Hela cell lysate with YPEL1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of YPEL1 in HeLa cells with YPEL1 antibody at 20 μg/mL.
Application
Immunocytochemistry of YPEL1 in HeLa cells with YPEL1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | YPEL1 antibody can be used for detection of YPEL1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | YPEL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | YPEL1 Antibody is supplied in PBS containing 0.02% sodium azide. |
