YPEL2 Antibody: YPEL2 (yippee-like 2) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL2 might be an important factor during the development and malignant transformation of tissues, most notably pancreatic and breast tumors.1) Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43.
Immunogen
YPEL2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human YPEL2. The immunogen is located within amino acids 30 - 80 of YPEL2.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICCIFWB
YPEL2 antibody can be used for detection of YPEL2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.:
Immunofluorescence of YPEL2 in HeLa cells with YPEL2 antibody at 5 μg/mL.
Application
Immunocytochemistry of YPEL2 in HeLa cells with YPEL2 antibody at 2.5 μg/mL.
Positive Control
1) Cat. No. 1201 - HeLa Cell Lysate
Application Notes
YPEL2 antibody can be used for detection of YPEL2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
YPEL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
YPEL2 Antibody is supplied in PBS containing 0.02% sodium azide.