Application
Western blot analysis of YPEL3 in A-20 cell lysate with YPEL3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptideApplication
Immunofluorescence of YPEL3 in A20 cells with YPEL3 antibody at 5 μg/mL.Application
Immunocytochemistry of YPEL3 in A20 cells with YPEL3 antibody at 2.5 μg/mL.| Product Name | YPEL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | YPEL3 Antibody: YPEL3 (yippee-like 3) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL3 is a p53-regulated gene whose expression is induced by DNA damage and in turn induces cellular senescence. It appears to function as a tumor suppressor as it is downregulated in colon and breast tumors.1) Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43. |
| Immunogen | YPEL3 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human YPEL3. The immunogen is located within amino acids 20 - 70 of YPEL3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,ICC,IF,WB |
| YPEL3 antibody can be used for detection of YPEL3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YPEL3 |
|---|---|
| Alternative Names | YPEL3 Antibody: Protein yippee-like 3 |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
Application
Western blot analysis of YPEL3 in A-20 cell lysate with YPEL3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Application
Immunofluorescence of YPEL3 in A20 cells with YPEL3 antibody at 5 μg/mL.
Application
Immunocytochemistry of YPEL3 in A20 cells with YPEL3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | YPEL3 antibody can be used for detection of YPEL3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | YPEL3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | YPEL3 Antibody is supplied in PBS containing 0.02% sodium azide. |
