Application
Western blot analysis of YPEL4 in SW480 cell lysate with YPEL4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of YPEL4 in SW480 cells with YPEL4 antibody at 5 μg/ml.Application
Immunocytochemistry of YPEL4 in SW480 cells with YPEL4 antibody at 2.5 μg/ml.| Product Name | YPEL4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YPEL4 Antibody: Protein yippee-like 4 |
| Product description | YPEL4 Antibody: YPEL4 (yippee-like 4) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL4 is ubiquitously expressed in adult tissues and has been shown to associate with the major vault protein (MVP). It has been suggested that MVP can inhibit YPEL4's ability to activate Elk-1 in the MAPK signaling pathway.1) Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43 |
| Immunogen | YPEL4 antibody was raised against a 12 amino acid synthetic peptide near the amino terminus of human YPEL4. The immunogen is located within the first 50 amino acids of YPEL4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| YPEL4 antibody can be used for detection of YPEL4 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YPEL4 |
|---|---|
| Alternative Names | YPEL4 Antibody: Protein yippee-like 4 |
| Sequence Similarities | 2) Cat. No. 17-207 - EL4 Cell Slide |
Application
Western blot analysis of YPEL4 in SW480 cell lysate with YPEL4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of YPEL4 in SW480 cells with YPEL4 antibody at 5 μg/ml.
Application
Immunocytochemistry of YPEL4 in SW480 cells with YPEL4 antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1221 - SW480 Cell Lysate |
|---|---|
| Application Notes | YPEL4 antibody can be used for detection of YPEL4 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | YPEL4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | YPEL4 Antibody is supplied in PBS containing 0.02% sodium azide. |
