Application
Western blot analysis of YPEL5 in A-20 cell lysate with YPEL5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of YPEL5 in A20 cells with YPEL5 antibody at 5 μg/mL.Application
Immunocytochemistry of YPEL5 in A20 cells with YPEL5 antibody at 2.5 μg/mL.| Product Name | YPEL5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YPEL5 Antibody: CGI-127, Protein yippee-like 5 |
| Product description | YPEL5 Antibody: YPEL5 (yippee-like 5) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL5 is expressed at the nucleus and centrosome during interphase; during mitosis, it localizes to the spindle poles, mitotic spindle, and spindle midzone during mitosis. Finally, during cytokinesis, YPEL5 is localized to the midbody. It is associated with the Ran Binding Protein in the Microtubule organizing center (RanBPM) and a related protein RanBP10.1) Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43. |
| Immunogen | YPEL5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human YPEL5. The immunogen is located within the first 50 amino acids of YPEL5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| YPEL5 antibody can be used for detection of YPEL5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YPEL5 |
|---|---|
| Alternative Names | YPEL5 Antibody: CGI-127 Protein yippee-like 5 |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
Application
Western blot analysis of YPEL5 in A-20 cell lysate with YPEL5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of YPEL5 in A20 cells with YPEL5 antibody at 5 μg/mL.
Application
Immunocytochemistry of YPEL5 in A20 cells with YPEL5 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | YPEL5 antibody can be used for detection of YPEL5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | YPEL5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | YPEL5 Antibody is supplied in PBS containing 0.02% sodium azide. |
