Application
Antibody is used in Western blot to detect YWHAB in HL-60 cell lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of YWHAB using rabbit polyclonal YWHAB Antibody (T71) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAB gene.| Product Name | YWHAB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha, N-terminally processed, YWHAB |
| Product description | YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.1) Komori, T., et al., Acta Neuropathol. 106(1):66-70 (2003). |
| Immunogen | This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human YWHAB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | YWHAB |
|---|---|
| Alternative Names | 14-3-3 protein beta/alpha Protein 1054 Protein kinase C inhibitor protein 1 KCIP-1 14-3-3 protein beta/alpha N-terminally processed YWHAB |
| Molecular Weight(MW) | 28 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Antibody is used in Western blot to detect YWHAB in HL-60 cell lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of YWHAB using rabbit polyclonal YWHAB Antibody (T71) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAB gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
