WB
Western blot analysis of extracts of various cell lines, using 14-3-3 al pha/beta antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.IHC
Immunohistochemistry of paraffin-embedded Human tonsil using 14-3-3 alpha/beta antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.ICC/IF
Immunofluorescence analysis of HeLa cells using 14-3-3 alpha/beta antibody at dilution of 1:100. Blue: DAPI for nuclear staining.| Product Name | 14-3-3 alpha/beta polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide of human 14-3-3 alpha/beta(NP_003395.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:100 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YWHAB |
|---|---|
| Gene Synonyms | HS1 GW128 YWHAA KCIP-1 HEL-S-1 |
| Gene Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta |
| Gene Summary | This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | YWHAB GW128 HEL-S-1 HS1 KCIP-1 YWHAA |
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Cytoplasm,Membrane,Vacuole. |
WB
Western blot analysis of extracts of various cell lines, using 14-3-3 al pha/beta antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
IHC
Immunohistochemistry of paraffin-embedded Human tonsil using 14-3-3 alpha/beta antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ICC/IF
Immunofluorescence analysis of HeLa cells using 14-3-3 alpha/beta antibody at dilution of 1:100. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:100 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
