Application
Western blot analysis using Dynamin1 monoclonal antibody against truncated ZAP70 recombinant protein and Jurkat cell lysate.Application
Immunohistochemical analysis of paraffin - embedded human lymph showing cytoplasmic localization using ZAP70 antibody with DAB staining.| Product Name | ZAP70 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRK, 70 kDa zeta-chain associated proteinSRK, STD, TZK, STCD, ZAP-70 |
| Product description | ZAP70(zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Zap70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the zap-70 gene. ZAP-70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.1) Sigal Gelkop , Gerrald D. Gish, Yael Babichev J Immunol. 2005 Dec 15;175(12):8123-32. |
| Immunogen | Ni-NTA purified truncated recombinant ZAP70 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ZAP70 |
|---|---|
| Alternative Names | SRK 70 kDa zeta-chain associated proteinSRK STD TZK STCD ZAP-70 |
Application
Western blot analysis using Dynamin1 monoclonal antibody against truncated ZAP70 recombinant protein and Jurkat cell lysate.
Application
Immunohistochemical analysis of paraffin - embedded human lymph showing cytoplasmic localization using ZAP70 antibody with DAB staining.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZAP70 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
