Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).Application
ZBBX antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZBBX Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Zinc finger B-box domain-containing protein 1, ZBBX |
| Product description | 1) Ota, T., et al. Nat. Genet. 36(1):40-45(2004) |
| Immunogen | This ZBBX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-62 amino acids from the N-terminal region of human ZBBX. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBBX |
|---|---|
| Alternative Names | Zinc finger B-box domain-containing protein 1 ZBBX |
| Molecular Weight(MW) | 91 kDa |
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Application
ZBBX antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
