Application
Western blot analysis of ZBED1 in A549 cell lysate with ZBED1 antibody at 1 μg/mL.| Product Name | ZBED1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBED1 Antibody: ALTE, DREF, TRAMP, hDREF, ALTE, KIAA0785, Zinc finger BED domain-containing protein 1, Putative Ac-like transposable element |
| Product description | ZBED1 Antibody: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.1) Ohshima N, Takahashi M, and Hirose F. Identification of a human homolog of the DREF transcription factor with a potential role in regulation of the histone H1 gene. J. Biol. Chem.2003; 278:22928-38. |
| Immunogen | ZBED1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ZBED1. The immunogen is located within amino acids 180 - 230 of ZBED1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBED1 |
|---|---|
| Alternative Names | ZBED1 Antibody: ALTE DREF TRAMP hDREF ALTE KIAA0785 Zinc finger BED domain-containing protein 1 Putative Ac-like transposable element |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3. |
Application
Western blot analysis of ZBED1 in A549 cell lysate with ZBED1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
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| Application Notes | ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBED1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBED1 Antibody is supplied in PBS containing 0.02% sodium azide. |
