Application
Western blot analysis of ZBED3 in Jurkat cell lysate with ZBED3 antibody at 1 μg/ml.Application
Immunocytochemistry of ZBED3 in A549 cells with ZBED3 antibody at 20 μg/mL.| Product Name | ZBED3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBED3 Antibody: Zinc finger BED domain-containing protein 3, Axin-interacting protein |
| Product description | ZBED3 Antibody: Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression.1) Chen T, Li M, Ding Y, et al. Identification of Zinc-finger BED domain containing 3 (Zbed3) as a novel axin-interacting protein that activates Wnt/b-catenin signaling. J. Biol. Chem.2009; 284:6683-9. |
| Immunogen | ZBED3 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ZBED3. The immunogen is located within the last 50 amino acids of ZBED3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 μg/mL. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBED3 |
|---|---|
| Alternative Names | ZBED3 Antibody: Zinc finger BED domain-containing protein 3 Axin-interacting protein |
| Molecular Weight(MW) | Predicted: 26 kDa Observed: 28 kDa |
| Sequence Similarities | 2) Cat. No. 17-003 - A549 Cell Slide |
| Tissue Specificity | This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2. |
Application
Western blot analysis of ZBED3 in Jurkat cell lysate with ZBED3 antibody at 1 μg/ml.
Application
Immunocytochemistry of ZBED3 in A549 cells with ZBED3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 μg/mL. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBED3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBED3 Antibody is supplied in PBS containing 0.02% sodium azide. |
