Application
Western blot analysis of ZBP1 in mouse small intestine tissue lysate with ZBP1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of ZBP1 in Human Small Intestine tissue with ZBP1 antibody at 20 μgg/mL.Application
Immunohistochemistry of ZBP1 in human small intestine tissue with ZBP1 antibody at 2.5 μg/mL.| Product Name | ZBP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBP1 Antibody: DAI, DLM1, DLM-1, C20orf183, Z-DNA-binding protein 1, Tumor stroma and activated macrophage protein DLM-1 |
| Product description | ZBP1 Antibody: Z-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Zalpha domain which binds specifically to left-handed Z-DNA and Z-RNA. ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia. More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK).1) Schwartz T, Behlke J, Lowenhaupt K, et al. Structure of the DLM-1-Z-DNA complex reveals a conserved family of Z-DNA-binding proteins. Nat. Struct. Biol. 2001; 8:761-5. |
| Immunogen | ZBP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1. The immunogen is located within the last 50 amino acids of ZBP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZBP1 antibody can be used for the detection of ZBP1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBP1 |
|---|---|
| Alternative Names | ZBP1 Antibody: DAI DLM1 DLM-1 C20orf183 Z-DNA-binding protein 1 Tumor stroma and activated macrophage protein DLM-1 |
| Molecular Weight(MW) | Predicted: 39, 45 kDa Observed: 42 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | Multiple isoforms of ZBP1 are known to exist. |
Application
Western blot analysis of ZBP1 in mouse small intestine tissue lysate with ZBP1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of ZBP1 in Human Small Intestine tissue with ZBP1 antibody at 20 μgg/mL.
Application
Immunohistochemistry of ZBP1 in human small intestine tissue with ZBP1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1408 - Mouse Small Intestine Tissue Lysate |
|---|---|
| Application Notes | ZBP1 antibody can be used for the detection of ZBP1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
