Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | ZBTB33 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBTB33, ZNF348, ZNF-kaiso |
| Product description | ZBTB33 is a transcriptional regulator with bimodal DNA-binding specificity. ZBTB33 binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway. It may also activate transcription of a subset of target genes by the recruitment of CTNND2.1) Defossez, P.A., (2005) J. Biol. Chem. 280 (52), 43017-43023. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZBTB33. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZBTB33 antibody can be used for detection of ZBTB33 by ELISA at 1:312500. ZBTB33 antibody can be used for detection of ZBTB33 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBTB33 |
|---|---|
| Alternative Names | ZBTB33 ZNF348 ZNF-kaiso |
| Molecular Weight(MW) | 74 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ZBTB33 antibody can be used for detection of ZBTB33 by ELISA at 1:312500. ZBTB33 antibody can be used for detection of ZBTB33 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZBTB33 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
