Application
Western blot analysis of ZBTB4 in SK-N-SH cell lysate with ZBTB4 antibody at 1 μg/mL.Application
Immunofluorescence of ZBTB4 in human brain tissue with ZBTB4 antibody at 20 μg/mL.Application
Immunohistochemistry of ZBTB4 in human brain tissue with ZBTB4 antibody at 2.5 μg/mL.| Product Name | ZBTB4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZBTB4 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.1) Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903. |
| Immunogen | ZBTB4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZBTB4. The immunogen is located within amino acids 790 - 840 of ZBTB4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,IHC-P,WB |
| ZBTB4 antibody can be used for detection of ZBTB4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBTB4 |
|---|---|
| Alternative Names | ZBTB4 Antibody: ZNF903 KAISO-L1 KIAA1538 Zinc finger and BTB domain-containing protein 4 KAISO-like zinc finger protein 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least four isoforms of ZBTB4 are known to exist; this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members. |
Application
Western blot analysis of ZBTB4 in SK-N-SH cell lysate with ZBTB4 antibody at 1 μg/mL.
Application
Immunofluorescence of ZBTB4 in human brain tissue with ZBTB4 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZBTB4 in human brain tissue with ZBTB4 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | ZBTB4 antibody can be used for detection of ZBTB4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBTB4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBTB4 Antibody is supplied in PBS containing 0.02% sodium azide. |
