Application
Western blot analysis of ZBTB7A in human ovary tissue lysate with ZBTB7A antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ZBTB7A in human ovary tissue with ZBTB7A antibody at 20 μg/mL.Application
Immunohistochemistry of ZBTB7A in human ovary tissue with ZBTB7A antibody at 2.5 μg/mL.| Product Name | ZBTB7A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBTB7A Antibody: LRF, FBI1, FBI-1, ZBTB7, ZNF857A, pokemon, LRF, Zinc finger and BTB domain-containing protein 7A, Factor binding IST protein 1 |
| Product description | ZBTB7A Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB7A, also known as LRF, was initially identified as a potential target of the LAZ-3/BCL-6 oncogene. Later reports demonstrated that it could bind to the wild-type IST (inducer of short transcripts) elements of HIV-1 long terminal repeats (LTRs) and stimulate Tat activity on the HIV-1 LTR. More recently it ZBTB7A has been recognized as a proto-oncogene whose overexpression contributes to malignancy in breast cancer and functions as a co-repressor of the androgen receptor in prostate cancer cells.1) Filion GJP, Zhenilo S, Salozhin S, et al. A family of zinc finger proteins that bind methylated DNA and repress transcription. Mol. Cell. Biol.2006; 26:169-81. |
| Immunogen | ZBTB7A antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZBTB7A. The immunogen is located within the first 50 amino acids of ZBTB7A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZBTB7A antibody can be used for detection of ZBTB7A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBTB7A |
|---|---|
| Alternative Names | ZBTB7A Antibody: LRF FBI1 FBI-1 ZBTB7 ZNF857A pokemon LRF Zinc finger and BTB domain-containing protein 7A Factor binding IST protein 1 |
| Sequence Similarities | 2) Cat. No. 11-201 - Human Ovary Tissue Slide |
| Tissue Specificity | At least two isoforms of ZBTB7A are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members. |
Application
Western blot analysis of ZBTB7A in human ovary tissue lysate with ZBTB7A antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ZBTB7A in human ovary tissue with ZBTB7A antibody at 20 μg/mL.
Application
Immunohistochemistry of ZBTB7A in human ovary tissue with ZBTB7A antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1316 - Human Ovary Tissue Lysate |
|---|---|
| Application Notes | ZBTB7A antibody can be used for detection of ZBTB7A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBTB7A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBTB7A Antibody is supplied in PBS containing 0.02% sodium azide. |
