WB
Western blot analysis of ZBTB7A on different lysates with Rabbit anti-ZBTB7A antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ZBTB7A antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling ZBTB7A with Rabbit anti-ZBTB7A antibody at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZBTB7A antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IP
ZBTB7A was immunoprecipitated from 0.2 mg HeLa cell lysate with ZBTB7A Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ZBTB7A Recombinant Rabbit Monoclonal Antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP, VHH(HRP) at 1/5,000 dilution was used for 1 hour at room temperature. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute;| Product Name | ZBTB7A Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000 IHC:1:200-1:1000 ICC/IF:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZBTB7A |
|---|---|
| Gene Synonyms | LRF FBI1 FBI-1 TIP21 ZBTB7 MNDLFH ZNF857A pokemon |
| Gene Full Name | zinc finger and BTB domain containing 7A |
| Gene Summary | Enables several functions, including SMAD binding activity; nuclear androgen receptor binding activity; and transcription corepressor binding activity. Involved in several processes, including erythrocyte maturation; negative regulation of signal transduction; and regulation of nucleobase-containing compound metabolic process. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | DKFZp547O146 antibody Factor binding IST protein 1 antibody Factor that binds to inducer of short transcripts protein 1 antibody FBI-1 antibody FBI1 antibody HIV-1 1st-binding protein 1 antibody HIV-1 inducer of short transcripts binding protein antibody HIV-1 inducer of short transcripts-binding factor 1 antibody Leukemia/lymphoma-related factor antibody LRF antibody lymphoma related factor antibody MGC99631 antibody POK erythroid myeloid ontogenic factor antibody Pokemon antibody POZ and Krueppel erythroid myeloid ontogenic factor antibody TIP21 antibody TTF-I-interacting peptide 21 antibody ZBT7A_HUMAN antibody ZBTB7 antibody ZBTB7A antibody Zinc finger and BTB domain containing 7A antibody zinc finger and BTB domain containing 7A HIV-1 inducer of short transcripts binding protein antibody Zinc finger and BTB domain-containing protein 7A antibody Zinc finger protein 857A antibody Zinc finger- and BTB domain-containing protein 7 antibody ZNF857A antibody |
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of ZBTB7A on different lysates with Rabbit anti-ZBTB7A antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ZBTB7A antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling ZBTB7A with Rabbit anti-ZBTB7A antibody at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZBTB7A antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IP
ZBTB7A was immunoprecipitated from 0.2 mg HeLa cell lysate with ZBTB7A Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ZBTB7A Recombinant Rabbit Monoclonal Antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP, VHH(HRP) at 1/5,000 dilution was used for 1 hour at room temperature. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute;| Application Notes | WB:1:1000 IHC:1:200-1:1000 ICC/IF:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
