Application
Western blot analysis of ZBTB8 in mouse spleen tissue lysate with ZBTB8 antibody at 1 μg/mL.Application
Immunofluorescence ofZBTB8 in human spleen tissue with ZBTB8 antibody at 20 μg/mL.Application
Immunohistochemistry of ZBTB8 in human spleen tissue with ZBTB9 antibody at 2.5 μg/mL.| Product Name | ZBTB8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBTB8 Antibody: BOZF1, ZBTB8, ZNF916A, BOZF1, Zinc finger and BTB domain-containing protein 8A, BOZ-F1 |
| Product description | ZBTB8 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB8, also known as BOZ-F1, is present in multiple tissues, and is highly expressed in the cytoplasm of the placenta. While little is known about ZBTB8, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB proteins, ZBTB7A, has been implicated as a proto-oncogene whose overexpression contributes to malignancy in breast cancer, suggesting that ZBTB8 may act as a transcriptional repressor or be involved in tumorigenesis.1) Filion GJP, Zhenilo S, Salozhin S, et al. A family of zinc finger proteins that bind methylated DNA and repress transcription. Mol. Cell. Biol.2006; 26:169-81. |
| Immunogen | ZBTB8 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB8. The immunogen is located within the last 50 amino acids of ZBTB8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZBTB8 antibody can be used for detection of ZBTB8 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBTB8A |
|---|---|
| Alternative Names | ZBTB8 Antibody: BOZF1 ZBTB8 ZNF916A BOZF1 Zinc finger and BTB domain-containing protein 8A BOZ-F1 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least two isoforms of ZBTB8 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members. |
Application
Western blot analysis of ZBTB8 in mouse spleen tissue lysate with ZBTB8 antibody at 1 μg/mL.
Application
Immunofluorescence ofZBTB8 in human spleen tissue with ZBTB8 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZBTB8 in human spleen tissue with ZBTB9 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
|---|---|
| Application Notes | ZBTB8 antibody can be used for detection of ZBTB8 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBTB8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBTB8 Antibody is supplied in PBS containing 0.02% sodium azide. |
