Application
Western blot analysis of ZC3H12A in K562 cell lysate with ZC3H12A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | ZC3H12A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZC3H12A Antibody: MCPIP, MCPIP1, dJ423B22.1, MCPIP, Ribonuclease ZC3H12A, MCP-induced protein 1 |
| Product description | ZC3H12A Antibody: ZC3H12A, also known as MCPIP, is an essential member of a family of novel CCCH-zinc finger proteins that regulate macrophage activation and may be involved in host immunity and inflammatory diseases. ZC3H12A has RNase activity that prevents some immune disorders by directly affecting the mRNA stability of interleukins such as IL-6 and IL12p40. Mice lacking the ZC3H12A gene suffered from severe anemia, and most dies within 12 weeks. Overexpression of ZC3H12A causes ER stress and induces a number of genes involved in apoptosis and autophagy, including JNK, PUMA, and beclin-1 in cardiac myoblasts, leading to cell death. ZC3H12A can also induce adipogenesis in 3T3-L1 pre-adipocytes in the absence of PPAPgamma, demonstrating the complex roles ZC3H12A plays.1) Zhou L, Azfer A, Niu J, et al. Monocyte chemoattractant protein-1 induces a novel transcription factor that causes cardiac myocyte apoptosis and ventricular dysfunction. Circ. Res. 2006; 98:1177-85. |
| Immunogen | ZC3H12A antibody was raised against a 17 amino acid synthetic peptide near the center of human ZC3H12A. The immunogen is located within amino acids 330 - 380 of ZC3H12A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZC3H12A antibody can be used for detection of ZC3H12A by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZC3H12A |
|---|---|
| Alternative Names | ZC3H12A Antibody: MCPIP MCPIP1 dJ423B22.1 MCPIP Ribonuclease ZC3H12A MCP-induced protein 1 |
| Molecular Weight(MW) | Predicted: 66 kDa Observed: 6 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (82%) |
| Tissue Specificity | This ZC3H12A antibody is predicted to have no cross-reactivity to other members of the ZC3H12 protein family. |
Application
Western blot analysis of ZC3H12A in K562 cell lysate with ZC3H12A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ZC3H12A antibody can be used for detection of ZC3H12A by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZC3H12A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide. |
