Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZC3H8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Zinc finger CCCH domain-containing protein 8, ZC3H8, ZC3HDC8 |
| Product description | Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.1) Lamesch, P., et al. Genomics 89(3):307-315(2007) Hwang, E.S., et al. Immunology 106(4):464-469(2002) Hwang, E.S., et al. J. Immunol. 169(1):248-253(2002) |
| Immunogen | This ZC3H8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human ZC3H8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZC3H8 |
|---|---|
| Alternative Names | Zinc finger CCCH domain-containing protein 8 ZC3H8 ZC3HDC8 |
| Molecular Weight(MW) | 34 kDa |
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
