Application
Western blot analysis of ZC3HAV1 in HeLa cell lysate with ZC3HAV1 antibody at 1μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of ZC3HAV1 in Hela cells with ZC3HAV1 antibody at 20 μg/mL.Application
Immunocytochemistry of ZC3HAV1 in HeLa cells with ZC3HAV1 antibody at 20 μg/mL.| Product Name | ZC3HAV1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZC3HAV1 Antibody: ZAP, ZC3H2, ARTD13, PARP13, FLB6421, ZC3HDC2, PRO1677, Zinc finger CCCH-type antiviral protein 1, ADP-ribosyltransferase diphtheria toxin-like 13 |
| Product description | ZC3HAV1 Antibody: The zinc finger antiviral protein (ZC3HAV1) is a CCCH type zinc finger protein that induces an innate immunity to infections by retrovirus by preventing the accumulation of viral RNAs in the cytoplasm and recruits the RNA processing exosome to degrade target RNAs, thereby inhibiting virus replication. ZC3HAV1 is localized in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. ZAP is a direct target gene of IRF3 action in cellular antiviral responses.1) Gao G, Guo X, and Goff SP. Inhibition of retroviral RNA production by ZAP, a CCCH-Type zinc finger protein. Science2002; 297:1703-6. |
| Immunogen | ZC3HAV1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human ZC3HAV1. The immunogen is located within amino acids 680 - 730 of ZC3HAV1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ZC3HAV1 antibody can be used for detection of ZC3HAV1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZC3HAV1 |
|---|---|
| Alternative Names | ZC3HAV1 Antibody: ZAP ZC3H2 ARTD13 PARP13 FLB6421 ZC3HDC2 PRO1677 Zinc finger CCCH-type antiviral protein 1 ADP-ribosyltransferase diphtheria toxin-like 13 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Western blot analysis of ZC3HAV1 in HeLa cell lysate with ZC3HAV1 antibody at 1μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of ZC3HAV1 in Hela cells with ZC3HAV1 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZC3HAV1 in HeLa cells with ZC3HAV1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ZC3HAV1 antibody can be used for detection of ZC3HAV1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZC3HAV1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZC3HAV1 Antibody is supplied in PBS containing 0.02% sodium azide. |
