Application
Western blot analysis in WiDr cell line lysates (35ug/lane).Application
ZC4H2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZC4H2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Zinc finger C4H2 domain-containing protein, Hepatocellular carcinoma-associated antigen 127, ZC4H2, HCA127, KIAA1166 |
| Product description | This protein contains a C4H2-type zinc finger and is thought to be involved in zinc ion binding. There are 3 isoforms produced by alternative splicing.1) Wu, C., et al. Proteomics 7(11):1775-1785(2007) |
| Immunogen | This ZC4H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-224 amino acids from the C-terminal region of human ZC4H2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZC4H2 |
|---|---|
| Alternative Names | Zinc finger C4H2 domain-containing protein Hepatocellular carcinoma-associated antigen 127 ZC4H2 HCA127 KIAA1166 |
| Molecular Weight(MW) | 26 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in WiDr cell line lysates (35ug/lane).
Application
ZC4H2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
