Application
Western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).Application
ZDHC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of ZDHC2 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Ramos cell line lysate.Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZDHC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Palmitoyltransferase ZDHHC2, Reduced expression associated with metastasis protein, Ream, Reduced expression in cancer protein, Rec, Zinc finger DHHC domain-containing protein 2, DHHC-2, Zinc finger protein 372, ZDHHC2, REAM, REC, ZNF372 |
| Product description | Palmitoyltransferase specific for GAP43 and DLG4/PSD95 (By similarity).1) Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009) |
| Immunogen | This ZDHC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the N-terminal region of human ZDHC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZDHHC2 |
|---|---|
| Alternative Names | Palmitoyltransferase ZDHHC2 Reduced expression associated with metastasis protein Ream Reduced expression in cancer protein Rec Zinc finger DHHC domain-containing protein 2 DHHC-2 Zinc finger protein 372 ZDHHC2 REAM REC ZNF372 |
| Molecular Weight(MW) | 42 kDa |
Application
Western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).
Application
ZDHC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of ZDHC2 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Ramos cell line lysate.
Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
